Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing MethodReportar como inadecuado


Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method


Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Singapore Centre for 3D Printing, School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore





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Academic Editor: Arne Berner

Abstract Polymeric fibrous scaffolds for guiding cell growth are designed to be potentially used for the tissue engineering TE of tubular organs including esophagi, blood vessels, tracheas, etc. Tubular scaffolds were fabricated via melt-drawing of highly elastic polyl-lactide-co-ε-caprolactone PLC fibers layer-by-layer on a cylindrical mandrel. The diameter and length of the scaffolds are customizable via 3D printing of the mandrel. Thickness of the scaffolds was varied by changing the number of layers of the melt-drawing process. The morphology and tensile properties of the PLC fibers were investigated. The fibers were highly aligned with a uniform diameter. Their diameters and tensile properties were tunable by varying the melt-drawing speeds. These tailorable topographies and tensile properties show that the additive-based scaffold fabrication technique is customizable at the micro- and macro-scale for different tubular tissues. The merits of these scaffolds in TE were further shown by the finding that myoblast and fibroblast cells seeded onto the scaffolds in vitro showed appropriate cell proliferation and distribution. Human mesenchymal stem cells hMSCs differentiated to smooth muscle lineage on the microfibrous scaffolds in the absence of soluble induction factors, showing cellular shape modulation and scaffold elasticity may encourage the myogenic differentiation of stem cells. View Full-Text

Keywords: tissue engineering; scaffold; additive manufacturing; tubular tissues; melt-drawing tissue engineering; scaffold; additive manufacturing; tubular tissues; melt-drawing





Autor: Yu Jun Tan * , Xipeng Tan, Wai Yee Yeong * and Shu Beng Tor

Fuente: http://mdpi.com/



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