Diversity of Nonribosomal Peptide Synthetase Genes in the Microbial Metagenomes of Marine SpongesReportar como inadecuado




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1

Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main St.W, Hamilton, ON L8S 4K1, Canada

2

Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs Platz 3, 97082 Würzburg, Germany



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Genomic mining revealed one major nonribosomal peptide synthetase NRPS phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely substrate. Additionally, a phylogenetically distinct NRPS gene cluster was discovered in the microbial metagenome of the sponge Aplysina aerophoba, which shows highest similarities to NRPS genes that were previously assigned, by ways of single cell genomics, to a Chloroflexi sponge symbiont. Genomic mining studies such as the one presented here for NRPS genes, contribute to on-going efforts to characterize the genomic potential of sponge-associated microbiota for secondary metabolite biosynthesis. View Full-Text

Keywords: nonribosomal peptide synthetase; NRPS; marine sponge; Porifera; metagenomics nonribosomal peptide synthetase; NRPS; marine sponge; Porifera; metagenomics





Autor: Sheila Marie Pimentel-Elardo 1,2,†, Lubomir Grozdanov 2,†, Sebastian Proksch 2 and Ute Hentschel 2,*

Fuente: http://mdpi.com/



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