SD118-Xanthocillin X 1, a Novel Marine Agent Extracted from Penicillium commune, Induces Autophagy through the Inhibition of the MEK-ERK PathwayReportar como inadecuado




SD118-Xanthocillin X 1, a Novel Marine Agent Extracted from Penicillium commune, Induces Autophagy through the Inhibition of the MEK-ERK Pathway - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China

2

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

3

PET Positron Emission Computed Tomography Center, General Hospital of Chengdu Military Command, Chengdu, Sichuan 610083, China



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Abstract A compound named SD118-xanthocillin X 1 C18H12N2O2, isolated from Penicillium commune in a deep-sea sediment sample, has been shown to inhibit the growth of several cancer cell lines in vitro. In the present study, we employed a growth inhibition assay and apoptotic analysis to identify the biological effect and detailed mechanism of SD118-xanthocillin X 1 in human hepatocellular carcinoma HepG2 cells. SD118-xanthocillin X 1 demonstrated a concentration-dependent inhibitory effect on the growth of HepG2 cells and caused slight cellular apoptosis and significantly induced autophagy. Autophagy was detected as early as 12 h by the conversion of microtubule-associated protein 1 light chain 3 LC3-I to LC3-II, following cleavage and lipid addition to LC3-I. The pharmacological autophagy inhibitor 3-methyladenine largely attenuates the growth inhibition and autophagic effect of SD118-xanthocillin X 1 in HepG2 cells. Our data also indicated that the autophagic effect of SD118-xanthocillin X 1 occurs via the down-regulation of the MEK-ERK signaling pathway and the up-regulated class III PI3K-Beclin 1 signaling pathway. View Full-Text

Keywords: SD118-xanthocillin X 1; autophagy; apoptosis; ERK; Beclin 1 SD118-xanthocillin X 1; autophagy; apoptosis; ERK; Beclin 1





Autor: Ying Zhao 1,†, Huan Chen 1,†, Zhuo Shang 2, Binghua Jiao 1, Bin Yuan 1, Weizhang Sun 3, Bingui Wang 2,* , Mingyong Miao 1,* and Caiguo Huang 1,*

Fuente: http://mdpi.com/



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