Krill Oil-In-Water Emulsion Protects against Lipopolysaccharide-Induced Proinflammatory Activation of Macrophages In VitroReportar como inadecuado


Krill Oil-In-Water Emulsion Protects against Lipopolysaccharide-Induced Proinflammatory Activation of Macrophages In Vitro


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1

Department of Medical Cell Biology, Philipps-University Marburg, Robert-Koch-Straße 8, 35032 Marburg, Germany

2

Stable Solutions LLC, Easton Industrial Park, 19 Norfolk Avenue, South Easton, MA 02375, USA

3

Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Sylvia Urban

Abstract Background: Parenteral nutrition is often a mandatory therapeutic strategy for cases of septicemia. Likewise, therapeutic application of anti-oxidants, anti-inflammatory therapy, and endotoxin lowering, by removal or inactivation, might be beneficial to ameliorate the systemic inflammatory response during the acute phases of critical illness. Concerning anti-inflammatory properties in this setting, omega-3 fatty acids of marine origin have been frequently described. This study investigated the anti-inflammatory and LPS-inactivating properties of krill oil KO-in-water emulsion in human macrophages in vitro. Materials and Methods: Differentiated THP-1 macrophages were activated using specific ultrapure-LPS that binds only on the toll-like receptor 4 TLR4 in order to determine the inhibitory properties of the KO emulsion on the LPS-binding capacity, and the subsequent release of TNF-α. Results: KO emulsion inhibited the macrophage binding of LPS to the TLR4 by 50% at 12.5 µg-mL and 75% at 25 µg-mL, whereas, at 50 µg-mL, completely abolished the LPS binding. Moreover, KO 12.5 µg-mL, 25 µg-mL, or 50 µg-mL also inhibited 30%, 40%, or 75%, respectively the TNF-α release after activation with 0.01 µg-mL LPS in comparison with LPS treatment alone. Conclusion: KO emulsion influences the LPS-induced pro-inflammatory activation of macrophages, possibly due to inactivation of the LPS binding capacity. View Full-Text

Keywords: krill oil-in-water emulsion; omega-3 fatty acids; phospholipids; LPS; cytokines; septic shock krill oil-in-water emulsion; omega-3 fatty acids; phospholipids; LPS; cytokines; septic shock





Autor: Gabriel A. Bonaterra 1,* , David Driscoll 2,3, Hans Schwarzbach 1 and Ralf Kinscherf 1

Fuente: http://mdpi.com/



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