Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 ProteinReportar como inadecuado


Biosynthesis of Polyunsaturated Fatty Acids in Octopus vulgaris: Molecular Cloning and Functional Characterisation of a Stearoyl-CoA Desaturase and an Elongation of Very Long-Chain Fatty Acid 4 Protein


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1

Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK

2

School of Applied Sciences, Edinburgh Napier University, Edinburgh EH11 4BN, Scotland, UK

3

Instituto de Agroquímica y Tecnología de Alimentos IATA-CSIC, Paterna, Valencia 46980, Spain

4

Instituto de Acuicultura Torre de la Sal IATS-CSIC, Ribera de Cabanes, Castellón 12595, Spain





*

Author to whom correspondence should be addressed.



Academic Editors: Rosário Domingues, Ricardo Calado and Pedro Domingues

Abstract Polyunsaturated fatty acids PUFAs have been acknowledged as essential nutrients for cephalopods but the specific PUFAs that satisfy the physiological requirements are unknown. To expand our previous investigations on characterisation of desaturases and elongases involved in the biosynthesis of PUFAs and hence determine the dietary PUFA requirements in cephalopods, this study aimed to investigate the roles that a stearoyl-CoA desaturase Scd and an elongation of very long-chain fatty acid 4 Elovl4 protein play in the biosynthesis of essential fatty acids FAs. Our results confirmed the Octopus vulgaris Scd is a ∆9 desaturase with relatively high affinity towards saturated FAs with ≥ C18 chain lengths. Scd was unable to desaturate 20:1n-15 ∆520:1 suggesting that its role in the biosynthesis of non-methylene interrupted FAs NMI FAs is limited to the introduction of the first unsaturation at ∆9 position. Interestingly, the previously characterised ∆5 fatty acyl desaturase was indeed able to convert 20:1n-9 ∆1120:1 to ∆5,1120:2, an NMI FA previously detected in octopus nephridium. Additionally, Elovl4 was able to mediate the production of 24:5n-3 and thus can contribute to docosahexaenoic acid DHA biosynthesis through the Sprecher pathway. Moreover, the octopus Elovl4 was confirmed to play a key role in the biosynthesis of very long-chain >C24 PUFAs. View Full-Text

Keywords: biosynthesis; elongation of very long-chain fatty acids 4 protein; non-methylene-interrupted fatty acids; polyunsaturated fatty acids; Octopus vulgaris; stearoyl-CoA desaturase biosynthesis; elongation of very long-chain fatty acids 4 protein; non-methylene-interrupted fatty acids; polyunsaturated fatty acids; Octopus vulgaris; stearoyl-CoA desaturase





Autor: Óscar Monroig 1,* , Rosa de Llanos 2,3, Inmaculada Varó 4, Francisco Hontoria 4, Douglas R. Tocher 1, Sergi Puig 3 and Juan C. Navarro 4

Fuente: http://mdpi.com/



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