The Effect of Phloroglucinol, A Component of Ecklonia cava Extract, on Hepatic Glucose ProductionReportar como inadecuado


The Effect of Phloroglucinol, A Component of Ecklonia cava Extract, on Hepatic Glucose Production


The Effect of Phloroglucinol, A Component of Ecklonia cava Extract, on Hepatic Glucose Production - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

College of Pharmacy, Gachon Institute of Pharmaceutical Science, Gachon University, Yeonsu-gu, Incheon 21936, Korea

2

Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Yeonsu-gu, Incheon 21999, Korea

3

Gachon Medical Research Institute, Gil Hospital, Namdong-gu, Incheon 21565, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Keith B. Glaser

Abstract Phloroglucinol is a phenolic compound that is one of the major compounds in Ecklonia cava brown alga. It has many pharmacological activities, but its anti-diabetic effect is not yet fully explored. In this study, we investigated the effect of phloroglucinol on the control of blood glucose levels and the regulation of hepatic glucose production. Phloroglucinol significantly improved glucose tolerance in male C57BL-6J mice fed a high fat diet HFD and inhibited glucose production in mouse primary hepatocytes. The expression of phosphoenol pyruvate carboxykinase PEPCK and glucose-6-phosphatase mRNA and protein G6Pase, enzymes involved in gluconeogenesis, were inhibited in liver tissue from phloroglucinol-treated mice and in phloroglucinol-treated HepG2 cells. In addition, phloroglucinol treatment increased phosphorylated AMP-activated protein kinase AMPKα in HepG2 cells. Treatment with compound C, an AMPKα inhibitor, inhibited the increase of phosphorylated AMPKα and the decrease of PEPCK and G6Pase expression caused by phloroglucinol treatment. We conclude that phloroglucinol may inhibit hepatic gluconeogenesis via modulating the AMPKα signaling pathway, and thus lower blood glucose levels. View Full-Text

Keywords: Phloroglucinol; gluconeogenesis; PEPCK; G6Pase; AMPKα Phloroglucinol; gluconeogenesis; PEPCK; G6Pase; AMPKα





Autor: Ji-Young Yoon 1,†, Hojung Choi 1,2,† and Hee-Sook Jun 1,2,3,*

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados