In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In VivoReportar como inadecuado


In Vitro Antioxidant Activities of Enzymatic Hydrolysate from Schizochytrium sp. and Its Hepatoprotective Effects on Acute Alcohol-Induced Liver Injury In Vivo


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1

The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China

2

College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China





*

Author to whom correspondence should be addressed.



Academic Editors: Se-Kwon Kim and Anake Kijjoa

Abstract Schizochytrium protein hydrolysate SPH was prepared through stepwise enzymatic hydrolysis by alcalase and flavourzyme sequentially. The proportion of hydrophobic amino acids of SPH was 34.71%. The molecular weight MW of SPH was principally concentrated at 180–3000 Da 52.29%. SPH was divided into two fractions by ultrafiltration: SPH-I MW < 3 kDa and SPH-II MW > 3 kDa. Besides showing lipid peroxidation inhibitory activity in vitro, SPH-I exhibited high DPPH and ABTS radicals scavenging activities with IC50 of 350 μg-mL and 17.5 μg-mL, respectively. In addition, the antioxidant activity of SPH-I was estimated in vivo using the model of acute alcohol-induced liver injury in mice. For the hepatoprotective effects, oral administration of SPH-I at different concentrations 100, 300 mg-kg BW to the mice subjected to alcohol significantly decreased serum alanine aminotransferase ALT and aspartate aminotransferase AST activities and hepatic malondialdehyde MDA level compared to the untreated mice. Besides, SPH-I could effectively restore the hepatic superoxide dismutase SOD, catalase CAT, and glutathione peroxidase GSH-Px activities and glutathione GSH level. Results suggested that SPH was rich in biopeptides that could be exploited as antioxidant molecules against oxidative stress in human body. View Full-Text

Keywords: Schizochytrium; protein hydrolysate; antioxidant; hepatoprotective effects; alcohol-induced liver injury Schizochytrium; protein hydrolysate; antioxidant; hepatoprotective effects; alcohol-induced liver injury





Autor: Xixi Cai 1,2, Ana Yan 2, Nanyan Fu 1 and Shaoyun Wang 2,*

Fuente: http://mdpi.com/



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