Cloning and Functional Characterization of a Lycopene β-Cyclase from Macrophytic Red Alga Bangia fuscopurpureaReportar como inadecuado


Cloning and Functional Characterization of a Lycopene β-Cyclase from Macrophytic Red Alga Bangia fuscopurpurea


Cloning and Functional Characterization of a Lycopene β-Cyclase from Macrophytic Red Alga Bangia fuscopurpurea - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China

2

Jiangsu Institute of Oceanology and Marine Fisheries, Nantong 226007, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Tatsuya Sugawara

Abstract Lycopene cyclases cyclize the open ends of acyclic lycopene ψ,ψ-carotene into β- or ε-ionone rings in the crucial bifurcation step of carotenoid biosynthesis. Among all carotenoid constituents, β-carotene β,β-carotene is found in all photosynthetic organisms, except for purple bacteria and heliobacteria, suggesting a ubiquitous distribution of lycopene β-cyclase activity in these organisms. In this work, we isolated a gene BfLCYB encoding a lycopene β-cyclase from Bangia fuscopurpurea, a red alga that is considered to be one of the primitive multicellular eukaryotic photosynthetic organisms and accumulates carotenoid constituents with both β- and ε-rings, including β-carotene, zeaxanthin, α-carotene β,ε-carotene and lutein. Functional complementation in Escherichia coli demonstrated that BfLCYB is able to catalyze cyclization of lycopene into monocyclic γ-carotene β,ψ-carotene and bicyclic β-carotene, and cyclization of the open end of monocyclic δ-carotene ε,ψ-carotene to produce α-carotene. No ε-cyclization activity was identified for BfLCYB. Sequence comparison showed that BfLCYB shares conserved domains with other functionally characterized lycopene cyclases from different organisms and belongs to a group of ancient lycopene cyclases. Although B. fuscopurpurea also synthesizes α-carotene and lutein, its enzyme-catalyzing ε-cyclization is still unknown. View Full-Text

Keywords: Bangia fuscopurpurea; red algae; lycopene cyclase; carotenoid; metabolism Bangia fuscopurpurea; red algae; lycopene cyclase; carotenoid; metabolism





Autor: Tian-Jun Cao 1, Xing-Qi Huang 1, Yuan-Yuan Qu 1, Zhong Zhuang 1, Yin-Yin Deng 2,* and Shan Lu 1,*

Fuente: http://mdpi.com/



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