Morphogenetically-Active Barrier Membrane for Guided Bone Regeneration, Based on Amorphous PolyphosphateReportar como inadecuado


Morphogenetically-Active Barrier Membrane for Guided Bone Regeneration, Based on Amorphous Polyphosphate


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1

ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, 55128 Mainz, Germany

2

Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University, Johann Joachim Becher Weg 13, D-55099 Mainz, Germany





*

Authors to whom correspondence should be addressed.



Academic Editors: Allen Place, Paul Long, Rosemary Jagus and Joy Watts

Abstract We describe a novel regeneratively-active barrier membrane which consists of a durable electrospun polyε-caprolactone PCL net covered with a morphogenetically-active biohybrid material composed of collagen and inorganic polyphosphate polyP. The patch-like fibrous collagen structures are decorated with small amorphous polyP nanoparticles 50 nm formed by precipitation of this energy-rich and enzyme-degradable alkaline phosphatase polymer in the presence of calcium ions. The fabricated PCL-polyP-collagen hybrid mats are characterized by advantageous biomechanical properties, such as enhanced flexibility and stretchability with almost unaltered tensile strength of the PCL net. The polyP-collagen material promotes the attachment and increases the viability-metabolic activity of human mesenchymal stem cells compared to cells grown on non-coated mats. The gene expression studies revealed that cells, growing onto polyP-collagen coated mats show a significantly two-fold higher upregulation of the steady-state-expression of the angiopoietin-2 gene used as an early marker for wound healing than cells cultivated onto non-coated mats. Based on our results we propose that amorphous polyP, stabilized onto a collagen matrix, might be a promising component of functionally-active barrier membranes for guided tissue regeneration in medicine and dentistry. View Full-Text

Keywords: biologization; hernia repair; inorganic polyphosphate; collagen-inducing; polypropylene mesh; tensile strength-resistance; stromal cell-derived factor-1; MC3T3-E1 cells biologization; hernia repair; inorganic polyphosphate; collagen-inducing; polypropylene mesh; tensile strength-resistance; stromal cell-derived factor-1; MC3T3-E1 cells





Autor: Xiaohong Wang 1,* , Maximilian Ackermann 2, Meik Neufurth 1, Shunfeng Wang 1, Heinz C. Schröder 1 and Werner E. G. Müller 1,*

Fuente: http://mdpi.com/



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