Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic CellsReportar como inadecuado


Cereus sinensis Polysaccharide and Its Immunomodulatory Properties in Human Monocytic Cells


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Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China





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Academic Editors: Paul Long, Orazio Taglialatela-Scafati and Alejandro M. Mayer

Abstract In this study, the extraction conditions of the crude polysaccharide from Cereus sinensis were optimized by response surface methodology. The optimum extraction conditions were: a ratio of raw material to water volume of 1:80 g-mL; an extraction temperature of 72 °C; and an extraction time of 3 h. Then, a purified polysaccharide named Cereus sinensis polysaccharide-1 CSP-1 was obtained from the crude polysaccharide by the Diethylaminoethyl cellulose-52 DEAE-52 cellulose chromatography column and Sephadex G-100 column. The molecular weight and monosaccharide composition of CSP-1 was determined through Gel Permeation Chromatography GPC and Gas Chromatography–Mass Spectrometer GS–MS, respectively. The results showed that CSP-1 with an average molecular weight of 56,335 Da was composed of l-−-Fucose, d-+-Mannose, d-Glucose and mainly possessed 1→2, 1→2, 6, 1→4, and 1→4, 6 of glycosyl linkages. The immunomodulatory activities of CSP-1 were also evaluated using lipopolysaccharide LPS-induced human monocytic THP-1 cells. The results demonstrated that CSP-1 dose-dependently protected against LPS-induced toxicity, and CSP-1 significantly inhibited the Toll-like receptor 4 TLR-4 mRNA, myeloid differentiation factor 88 MyD88 mRNA and tumour necrosis factor receptor-associated factor-6 TRAF-6 mRNA expression of the LPS-induced THP-1 cells, as well as suppressing reactive oxygen species ROS generation. View Full-Text

Keywords: Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property Cereus sinensis; polysaccharide; THP-1 cells; immunomodulatory property





Autor: Junwen Wu, Xuefei Zhou, Min Zhang, Yun Yao, Juanjuan Han and Kehai Liu *

Fuente: http://mdpi.com/



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