Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical CharacterizationReportar como inadecuado


Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization


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1

Department of Pharmacognosy and Chemistry of Natural Products, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece

2

Institute of Nanosciences and Nanotechnology, NCSR -Demokritos-, Aghia Paraskevi 15310, Attiki, Greece

3

Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytoko Str., Nea Ionia 38445, Magnesia, Greece





*

Author to whom correspondence should be addressed.



Abstract In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively. View Full-Text

Keywords: Axinella cannabina; Suberites carnosus; sponges; marine collagen Axinella cannabina; Suberites carnosus; sponges; marine collagen





Autor: Leto-Aikaterini Tziveleka 1, Efstathia Ioannou 1, Dimitris Tsiourvas 2, Panagiotis Berillis 3, Evangelia Foufa 1 and Vassilios Roussis 1,*

Fuente: http://mdpi.com/



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