Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic FemoraReportar como inadecuado




Effects of Time of Initial Exposure to MSV Sarcoma on Bone Induction by Dentine Matrix Implants and on Orthotopic Femora - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Chair of Histology and Embryology, Institute of Biostructural Research, Medical University of Warsaw, Chalubinskiego 5 Str., 02-004 Warszawa, Poland

2

Department of Conservative Dentistry, Institute of Stomatology, Medical University of Warsaw, Miodowa 18 Str., 00-246 Warszawa, Poland





*

Author to whom correspondence should be addressed.



Abstract HCl-demineralized murine lower incisors were implanted intramuscularly into syngeneic BALB-c mice to induce heterotopic osteogenesis. Implants were exposed at the early, preosteogenic stage 4, or at the later, osteogenic stage 12 to the Moloney sarcoma virus MSV, which within 3–4 days results in a sarcoma. The yield of bone induction was determined by weight of dry bone mass following NaOH hydrolysis of soft tissues. To verify the effect of this sarcoma on orthotopic local femoral bone, the dry mass of the tumor-exposed femora was measured and compared with the weight of MSV-unexposed contralateral controls. MSV-sarcoma or cells involved with their spontaneous rejection have a stimulatory effect on the periosteal membrane of the tumor-adjacent femoral bones, increasing their dry mass on average by 18%. No stimulatory effect on heterotopic bone induction was observed when the MSV sarcoma grew during the early, preosteogenic stage 4 onward, but when the tooth matrix had been exposed to such tumor at the already bone-forming stage, 12 onward, the yield of bone induction was enhanced. Thus, it is postulated that lesions induced by MSV during the early, preosteogenic stage inhibit recruitment of osteoprogenitor cells or degrade Bone Morphogenetic Proteins BMPs released by matrix resorbing inflammatory cells, whereas when acting on already existing bone they have a stimulatory effect. View Full-Text

Keywords: ectopic osteogenesis; murine demineralized incisors; MSV sarcoma; periosteum activation ectopic osteogenesis; murine demineralized incisors; MSV sarcoma; periosteum activation





Autor: Krzysztof Włodarski 1,* , Paweł Włodarski 1, Ryszard Galus 1 and Aniela Brodzikowska 2

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados