The Mechanism of R,R ZX-5 on Increasing NO ReleaseReportar como inadecuado




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Department of Marine Pharmacy, College of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China

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Department of Pharmacy, University of China Pharmaceutical, Nanjing 210009, Jiangsu Province, China





*

Author to whom correspondence should be addressed.



Abstract R,R ZX-5 has been proven to have positive effects on choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits. This study was designed to investigate the mechanisms of R,R ZX-5 on improving the choroidal blood flow and promoting NO production. HUVECs human umbilical vein endothelial cells were used to determine the production of eNOS, p-eNOS, AKT and Erk1-2 by Western blot analysis. iNOS and eNOS mRNA levels were investigated by RT-PCR and the effect of R,R ZX-5 on NO production were determined by eNOS activity assay. We found R,R ZX-5 upregulated protein expression of eNOS and iNOS, increased NO production, and reduced ERK and Akt protein level. Therefore, R,R ZX-5 may promote the choroidal blood flow in New Zealand white rabbits without affecting the blood flow in the iris or ciliary bodies via increasing NO production. These results suggest that R,R ZX-5 may function to cure and prevent Age-related macular degeneration AMD. View Full-Text

Keywords: R,R ZX-5; iNOS; eNOS; ERK; Akt R,R ZX-5; iNOS; eNOS; ERK; Akt





Autor: Han-Mei Xu 1, Jin Wei 1, Li Pan 1, Hongying Lin 1, Weiqiang Wang 1, Yihua Zhang 2 and Zilong Shen 1,*

Fuente: http://mdpi.com/



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