In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1Reportar como inadecuado




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1

National Key Lab for Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

2

Guelph Food Research Center, Agriculture and Agri-Food Canada, Guelph N1G5C9, Canada



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Aflatoxin B1 AFB1 is commonly found in cereals and animal feeds and causes a significant threat to the food industry and animal production. Several microbial isolates with high AFB1 transformation ability have been identified in our previous studies. The aim of this research was to characterize one of those isolates, Myxococcus fulvus ANSM068, and to explore its biotransformation mechanism. The bacterial isolate of M. fulvus ANSM068, isolated from deer feces, was able to transform AFB1 by 80.7% in liquid VY-2 medium after incubation at 30 °C for 72 h. The supernatant of the bacterial culture was more effective in transforming AFB1 as compared to the cells alone and the cell extract. The transformation activity was significantly reduced and eradicated after the culture supernatant was treated with proteinase K, proteinase K plus SDS and heating. Culture conditions, including nitrogen source, initial pH and incubation temperature were evaluated for an optimal AFB1 transformation. Liquid chromatography mass spectrometry LCMS analyses showed that AFB1 was transformed to a structurally different compound. Infrared analysis IR indicated that the lactone ring on the AFB1 molecule was modified by the culture supernatant. Chromatographies on DEAE-Ion exchange and Sephadex-Molecular sieve and SDS-PAGE electrophoresis were used to determine active components from the culture supernatant, indicating that enzymes were responsible for the AFB1 biotransformation. This is the first report on AFB1 transformation by a strain of myxobacteria through enzymatic reactions. View Full-Text

Keywords: aflatoxin B1; biotransformation; culture supernatant; Myxococcus fulvus ANSM068 aflatoxin B1; biotransformation; culture supernatant; Myxococcus fulvus ANSM068





Autor: Shu Guan 1,2,†, Lihong Zhao 1,†, Qiugang Ma 1, Ting Zhou 2, Ning Wang 1, Xinxu Hu 1 and Cheng Ji 1,*

Fuente: http://mdpi.com/



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