2-Decenoic Acid Ethyl Ester, a Compound That Elicits Neurotrophin-like Intracellular Signals, Facilitating Functional Recovery from Cerebral Infarction in MiceReportar como inadecuado




2-Decenoic Acid Ethyl Ester, a Compound That Elicits Neurotrophin-like Intracellular Signals, Facilitating Functional Recovery from Cerebral Infarction in Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Laboratory of Molecular Biology, Gifu Pharmaceutical University, Daigaku-nishi, 1-25-4, Gifu 501-1190, Japan

2

Department of Neurosurgery, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu 501-1194, Japan





*

Author to whom correspondence should be addressed.



Abstract In our previous study, we found that trans-2-decenoic acid ethyl ester DAEE, a derivative of a medium-chain fatty acid, elicits neurotrophin-like signals including the activation of extracellular signal-regulated kinases 1 and 2 ERK1-2 in cultured mouse cortical neurons. Here, we examined the efficacy of intraperitoneal administration of DAEE on the treatment of a mouse model of the cerebral infarction caused by unilateral permanent middle cerebral artery occlusion PMCAO. DAEE-treatment 100 µg-kg body weight injected at 0.5, 24, 48, 72 h after PMCAO significantly restored the mice from PMCAO-induced neurological deficits including motor paralysis when evaluated 48, 72, and 96 h after the PMCAO. Furthermore, DAEE facilitated the phosphorylation of ERK1-2 on the infarction side of the brain when analyzed by Western immunoblot analysis, and it enhanced the number of phosphorylated ERK1-2-positive cells in the border areas between the infarction and non-infarction regions of the cerebral cortex, as estimated immunohistochemically. As the infarct volume remained unchanged after DAEE-treatment, it is more likely that DAEE improved the neurological condition through enhanced neuronal functions of the remaining neurons in the damaged areas rather than by maintaining neuronal survival. These results suggest that DAEE has a neuro-protective effect on cerebral infarction. View Full-Text

Keywords: 2-decenoic acid ethyl ester; neurotrophin; extracellular signal-regulated kinases 1 and 2; cerebral infarction 2-decenoic acid ethyl ester; neurotrophin; extracellular signal-regulated kinases 1 and 2; cerebral infarction





Autor: Yoshitaka Tanaka 1,2, Hidefumi Fukumitsu 1, Hitomi Soumiya 1, Shinichi Yoshimura 2, Toru Iwama 2 and Shoei Furukawa 1,*

Fuente: http://mdpi.com/



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