Exploration of the Role of the Non-Coding RNA SbrE in L. monocytogenes Stress ResponseReportar como inadecuado




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1

Department of Food Science, Cornell University, Ithaca, NY 14853, USA

2

Department of Food Science, Purdue University, West Lafayette, IN 47907, USA





*

Author to whom correspondence should be addressed.



Abstract SbrE is a ncRNA in Listeria monocytogenes, reported to be up-regulated by the alternative sigma factor σB. Initial quantitative RT-PCR qRT-PCR experiments on parent strains and isogenic ΔsigB strains demonstrated σB-dependent expression of SbrE across the four L. monocytogenes lineages and in L. innocua. Microarray and proteomics MDLC-MS-MS with iTRAQ labeling experiments with the L. monocytogenes parent strain and an isogenic ΔsbrE strain identified a single gene lmo0636 and two proteins Lmo0637 and Lmo2094 that showed lower expression levels in the ΔsbrE strain. qRT-PCR demonstrated an increase in SbrE transcript levels in stationary phase L. monocytogenes and in bacteria exposed to oxidative stress mean log2 transcript levels 7.68 ± 0.57 and 1.70 ± 0.71 greater than in mid-log phase cells, respectively. However, no significant differences in growth or survival between the parent strain and ΔsbrE strain were confirmed under a variety of environmental stress conditions tested. Our data suggest that σB-dependent transcription of SbrE represents a conserved mechanism that contributes, across Listeria species, to fine-tuning of gene expression under specific environmental conditions that remain to be defined. View Full-Text

Keywords: non-coding RNA; Listeria monocytogenes; sigma B non-coding RNA; Listeria monocytogenes; sigma B





Autor: Sana Mujahid 1, Teresa M. Bergholz 1, Haley F. Oliver 1,2, Kathryn J. Boor 1 and Martin Wiedmann 1,*

Fuente: http://mdpi.com/



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