TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces ApoptosisReportar como inadecuado




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1

Faculty of Pharmacy -Iuliu Hatieganu- University of Medicine and Pharmacy, 4 Pasteur Street, Cluj-Napoca 400349, Romania

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Department of Functional Genomics and Experimental Pathology, Cancer Institute -Ioan Chiricută-, 34–36 Republici Street, Cluj-Napoca 400015, Romania

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Research Center for Functional Genomics, Biomedicine and Translational Medicine -Iuliu Hatieganu- University of Medicine and Pharmacy, 8 Victor Babes Street, Cluj-Napoca 400012, Romania

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Surgical Clinic II, 4–6 Clinicilor Street, Cluj-Napoca 400006, Romania

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Department of Surgery -Iuliu Haţieganu- University of Medicine and Pharmacy, 8 Victor Babes Street, Cluj-Napoca 400012, Romania





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Author to whom correspondence should be addressed.



Abstract Tumor necrosis factor alpha TNF-α is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experiments, we used as an in vitro model for triple negative breast cancer the cell line Hs578T. The purpose of this study is to determine the gene expression profiling of apoptotic signaling networks after blocking TNF-α formation by using specially designed siRNA molecules to target TNF-α messenger RNA. Knockdown of TNF-α gene was associated with cell proliferation inhibition and apoptosis, as observed by monitoring the cell index using the xCELLigence RTCA System and flow cytometry. PCR array technology was used to examine the transcript levels of 84 genes involved in apoptosis. 15 genes were found to be relevant after comparing the treated group with the untreated one of which 3 were down-regulated and 12 up-regulated. The down-regulated genes are all involved in cell survival, whereas the up-regulated ones are involved in and interact with pro-apoptotic pathways. The results described here indicate that the direct target of TNF-α in the Hs578T breast cancer cell line increases the level of certain pro-apoptotic factors that modulate different cellular networks that direct the cells towards death. View Full-Text

Keywords: RNA interference; apoptosis; cell signaling pathways; gene therapy RNA interference; apoptosis; cell signaling pathways; gene therapy





Autor: Valentina Pileczki 1, Cornelia Braicu 2,3, Claudia D. Gherman 4,5,* and Ioana Berindan-Neagoe 2,3

Fuente: http://mdpi.com/



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