Ultraviolet B UVB Irradiation-Induced Apoptosis in Various Cell Lineages in VitroReportar como inadecuado




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1

DiSTeVA, University of Urbino -Carlo Bo-, Urbino 61029, Italy

2

Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopaedic Institute, Bologna 40136, Italy

3

IGM, CNR, Rizzoli Orthopaedic Institute, Bologna 40136, Italy





*

Author to whom correspondence should be addressed.



Abstract Ultraviolet B UVB radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. Several studies have demonstrated that UVB-induced cell death occurs through the generation of reactive oxygen species. The consequent oxidative stress includes the impairment of cellular antioxidants, the induction of DNA damage and the occurrence of apoptosis. In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. Myeloid leukemia HL-60, T-lymphoblastoid Molt-4 and myelomonocytic U937 human cells, generally affected by apoptotic stimuli, were studied. Human chondrocytes and C2C12 skeletal muscle cells, known to be more resistant to damage, were also considered. All of them, when exposed to UVB radiation, revealed a number of characteristic apoptotic markers. Membrane blebbing, cytoplasm shrinkage and chromatin condensation were detected by means of electron microscopy. DNA cleavage, investigated by using agarose gel electrophoresis and TUNEL reaction, was observed in suspended cells. Differently, in chondrocytes and in skeletal muscle cells, oligonucleosomic DNA fragmentation did not appear, even if a certain TUNEL positivity was detected. These findings demonstrate that UVB radiation appears to be an ideal tool to study the apoptotic behavior. View Full-Text

Keywords: UVB; apoptosis; HL-60; Molt-4; U937; chondrocytes; skeletal muscle cells UVB; apoptosis; HL-60; Molt-4; U937; chondrocytes; skeletal muscle cells





Autor: Sara Salucci 1, Sabrina Burattini 1, Michela Battistelli 1, Valentina Baldassarri 1, Maria Cristina Maltarello 2 and Elisabetta Falcieri 1,3,*

Fuente: http://mdpi.com/



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