Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA-Rho Kinase PathwayReportar como inadecuado




Functional and Molecular Changes of the Bladder in Rats with Crushing Injury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Role of RhoA-Rho Kinase Pathway - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Urology, Seoul St. Marys Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea

2

Department of Urology, St. Vincents Hospital, the Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Gyeonggi-do, Korea





*

Author to whom correspondence should be addressed.



Abstract Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion MPG to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA-Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor. View Full-Text

Keywords: urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat urinary bladder; receptor; muscarinic; Rho-associated kinase; autonomic ganglia; injury; nerve crush; rat





Autor: Su Jin Kim 1, Dong Sup Lee 2, Woong Jin Bae 1, Seol Kim 1, Sung Hoo Hong 1, Ji Youl Lee 1, Tae-Kon Hwang 1 and Sae Woong Kim 1,*

Fuente: http://mdpi.com/



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