Progressive Changes in Inflammatory and Matrix Adherence of Bronchial Epithelial Cells with Persistent Respiratory Syncytial Virus RSV Infection Progressive Changes in RSV InfectionReportar como inadecuado




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1

Department of Physiology, School of Basic Medical Science, Central South University, Changsha 410078, China

2

Department of Physiology, Guangzhou Medical University, Guangzhou 510182, China

3

Respiratory Department, Xiangya Hospital, Central South University, Changsha 410078, China





*

Author to whom correspondence should be addressed.



Abstract In addition to the acute manifestations of respiratory syncytial virus RSV, persistent infection may be associated with long-term complications in the development of chronic respiratory diseases. To understand the mechanisms underlying RSV-induced long-term consequences, we established an in vitro RSV strain A2 infection model using human bronchial epithelial 16HBE cells that persists over four generations and analyzed cell inflammation and matrix adherence. Cells infected with RSV at multiplicity of infection MOI 0.0067 experienced cytolytic or abortive infections in the second generation G2 or G3 but mostly survived up to G4. Cell morphology, leukocyte and matrix adherence of the cells did not change in G1 or G2, but subsequently, leukocyte adherence and cytokine-chemokine secretion, partially mediated by intercellular adhesion molecule-1 ICAM-1, increased drastically, and matrix adherence, partially mediated by E-cadherin, decreased until the cells died. Tumor necrosis factor-α TNF-α secretion was inhibited by ICAM-1 antibody in infected-16HBE cells, suggesting that positive feedback between TNF-α secretion and ICAM-1 expression may be significant in exacerbated inflammation. These data demonstrate the susceptibility of 16HBE cells to RSV and their capacity to produce long-term progressive RSV infection, which may contribute to inflammation mobilization and epithelial shedding. View Full-Text

Keywords: respiratory syncytial virus; human bronchial epithelial cells; adherence; adhesive molecule; cytokine; chemokine respiratory syncytial virus; human bronchial epithelial cells; adherence; adhesive molecule; cytokine; chemokine





Autor: Xiaoai Liu 1,2, Xiaoqun Qin 1,* , Yang Xiang 1, Huijun Liu 1, Ge Gao 1, Ling Qin 3, Chi Liu 1 and Xiangping Qu 1

Fuente: http://mdpi.com/



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