X-Ray Induced DNA Damage and Repair in Germ Cells of PARP1−-− Male MiceReportar como inadecuado




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1

Unit of Radiation Biology and Human Health, Italian National Agency for New Technologies, Energy and Sustainable Economic Development ENEA, CR Casaccia, Via Anguillarese 301, Roma 00123, Italy

2

Department of Ecology and Biology, University of Tuscia, Viterbo 01100, Italy





*

Author to whom correspondence should be addressed.



Abstract PolyADP-ribosepolymerase-1 PARP1 is a nuclear protein implicated in DNA repair, recombination, replication, and chromatin remodeling. The aim of this study was to evaluate possible differences between PARP1−-− and wild-type mice regarding induction and repair of DNA lesions in irradiated male germ cells. Comet assay was applied to detect DNA damage in testicular cells immediately, and two hours after 4 Gy X-ray irradiation. A similar level of spontaneous and radiation-induced DNA damage was observed in PARP1−-− and wild-type mice. Conversely, two hours after irradiation, a significant level of residual damage was observed in PARP1−-− cells only. This finding was particularly evident in round spermatids. To evaluate if PARP1 had also a role in the dynamics of H2AX phosphorylation in round spermatids, in which γ-H2AX foci had been shown to persist after completion of DNA repair, we carried out a parallel analysis of γ-H2AX foci at 0.5, 2, and 48 h after irradiation in wild-type and PARP1−-− mice. No evidence was obtained of an effect of PARP1 depletion on H2AX phosphorylation induction and removal. Our results suggest that, in round spermatids, under the tested experimental conditions, PARP1 has a role in radiation-induced DNA damage repair rather than in long-term chromatin modifications signaled by phosphorylated H2AX. View Full-Text

Keywords: polyADP-ribosepolymerase-1; DNA repair; male mouse germ cells; comet assay; H2AX phosphorylation; ionizing radiation polyADP-ribosepolymerase-1; DNA repair; male mouse germ cells; comet assay; H2AX phosphorylation; ionizing radiation





Autor: Paola Villani 1,* , Anna Maria Fresegna 1, Roberto Ranaldi 1, Patrizia Eleuteri 1, Lorena Paris 1,2, Francesca Pacchierotti 1 and Eugenia Cordelli 1

Fuente: http://mdpi.com/



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