Neuroprotective Effects of α-Tocotrienol on Kainic Acid-Induced Neurotoxicity in Organotypic Hippocampal Slice CulturesReportar como inadecuado




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1

Department of Physiology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea

2

Department of Dental Hygiene, Division of Health Science, Dongseo University, Busan 617-716, Korea

3

Department of Biomedical Laboratory Science, Division of Health Science, Dongseo University, Busan 617-716, Korea





*

Author to whom correspondence should be addressed.



Abstract Vitamin E, such as alpha-tocopherol ATPH and alpha-tocotrienol ATTN, is a chain-breaking antioxidant that prevents the chain propagation step during lipid peroxidation. In the present study, we investigated the effects of ATTN on KA-induced neuronal death using organotypic hippocampal slice culture OHSC and compared the neuroprotective effects of ATTN and ATPH. After 15 h KA 5 µM treatment, delayed neuronal death was detected in the CA3 region and reactive oxygen species ROS formation and lipid peroxidation were also increased. Both co-treatment and post-treatment of ATPH 100 µM or ATTN 100 µM significantly increased the cell survival and reduced the number of TUNEL-positive cells in the CA3 region. Increased dichlorofluorescein DCF fluorescence and levels of thiobarbiturate reactive substances TBARS were decreased by ATPH and ATTN treatment. These data suggest that ATPH and ATTN treatment have protective effects on KA-induced cell death in OHSC. ATTN treatment tended to be more effective than ATPH treatment, even though there was no significant difference between ATPH and ATTN in co-treatment or post-treatment. View Full-Text

Keywords: kainic acid; organotypic hippocampal slice culture; antioxidant; alpha-tocopherol; alpha-tocotrienol kainic acid; organotypic hippocampal slice culture; antioxidant; alpha-tocopherol; alpha-tocotrienol





Autor: Na Young Jung 1, Kyung Hee Lee 2, Ran Won 3 and Bae Hwan Lee 1,*

Fuente: http://mdpi.com/



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