Design of Phosphonium-Type Zwitterion as an Additive to Improve Saturated Water Content of Phase-Separated Ionic Liquid from Aqueous Phase toward Reversible Extraction of ProteinsReportar como inadecuado




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1

Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan

2

Functional Ionic Liquid Laboratories, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan

3

Japan Science and technology Agency JST, Core Research for Evolutional Science and Technology CREST, Chiyoda, Tokyo 102-0076, Japan





*

Author to whom correspondence should be addressed.



Abstract We designed phosphonium-type zwitterion ZI to control the saturated water content of separated ionic liquid IL phase in the hydrophobic IL-water biphasic systems. The saturated water content of separated IL phase, 1-butyl-3-methyimidazolium bistrifluoromethanesulfonylimide, was considerably improved from 0.4 wt% to 62.8 wt% by adding N,N,N-tripentyl-4-sulfonyl-1-butanephosphonium-type ZI P555C4S. In addition, the maximum water content decreased from 62.8 wt% to 34.1 wt% by increasing KH2PO4-K2HPO4 salt content in upper aqueous phosphate buffer phase. Horse heart cytochrome c cyt.c was dissolved selectively in IL phase by improving the water content of IL phase, and spectroscopic analysis revealed that the dissolved cyt.c retained its higher ordered structure. Furthermore, cyt. c dissolved in IL phase was re-extracted again from IL phase to aqueous phase by increasing the concentration of inorganic salts of the buffer solution. View Full-Text

Keywords: ionic liquid-water biphasic system; protein extraction; zwitterion; hydrophobicity; recovering ionic liquid-water biphasic system; protein extraction; zwitterion; hydrophobicity; recovering





Autor: Yoritsugu Ito 1,2, Yuki Kohno 1,2, Nobuhumi Nakamura 1,2,3 and Hiroyuki Ohno 1,2,3,*

Fuente: http://mdpi.com/



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