Photochemistry and Cytotoxicity Evaluation of Heptamethinecyanine Near Infrared NIR DyesReportar como inadecuado




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Molecular Physical Chemistry Center, and IN-Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Technical University of Lisbon, Av. Rovisco Pais, 1049-001 Lisboa, Portugal





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Abstract The present study investigates the photochemical properties of potential photosensitizers for photodynamic therapy, namely four commercial heptamethinecyanine dyes IR125, IR780, IR813, IR820. Spectroscopic studies were made by means of laser induced fluorescence and laser flash photolysis in order to obtain fluorescence quantum yields and transient absorption spectra. Fluorescence lifetimes were also determined. The differences encountered were essentially related with the interaction of the sulfonate groups with the solvent, and also regarding the rigidification of the central bridge connecting the two nitrogen-containing heterocyclic groups. Transient absorption studies were performed both in aerated and oxygen free samples, to conclude about the formation of photoisomers and triplet state. For the four dyes under study, a cytotoxic evaluation in the dark and after irradiation was performed using HeLa cells as the model cell line, which revealed significant changes after irradiation mainly in IR125 and IR813 dyes. Confocal microscopy analysis showed that these dyes tend to enter to the intracellular space. View Full-Text

Keywords: heptamethine cyanine dyes; fluorescence quantum yields and lifetimes; transient absorption; PDT; HeLa cells; photo and dark cytotoxicity; confocal microscopy heptamethine cyanine dyes; fluorescence quantum yields and lifetimes; transient absorption; PDT; HeLa cells; photo and dark cytotoxicity; confocal microscopy





Autor: David S. Conceição, Diana P. Ferreira and Luís F. Vieira Ferreira *

Fuente: http://mdpi.com/



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