MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1-AKT-mTOR Signal PathwayReportar como inadecuado

MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1-AKT-mTOR Signal Pathway - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China

These authors contributed equally to this work.


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Abstract MicroRNAs constitute a class of ~22-nucleotide non-coding RNAs. They modulate gene expression by associating with the 3 untranslated regions 3 UTRs of messenger RNAs mRNAs. Although multiple miRNAs are known to be regulated during myoblast differentiation, their individual roles in muscle development are still not fully understood. In this study, we showed that miR-199a-3p was highly expressed in skeletal muscle and was induced during C2C12 myoblasts differentiation. We also identified and confirmed several genes of the IGF-1-AKT-mTOR signal pathway, including IGF-1, mTOR, and RPS6KA6, as important cellular targets of miR-199a-3p in myoblasts. Overexpression of miR-199a-3p partially blocked C2C12 myoblast differentiation and the activation of AKT-mTOR signal pathway, while interference of miR-199a-3p by antisense oligonucleotides promoted C2C12 differentiation and myotube hypertrophy. Thus, our studies have established miR-199a-3p as a potential regulator of myogenesis through the suppression of IGF-1-AKT-mTOR signal pathway. View Full-Text

Keywords: miR-199a-3p; C2C12; muscle; myogenic differentiation miR-199a-3p; C2C12; muscle; myogenic differentiation

Autor: Long Jia †, Yue-Feng Li †, Guo-Fang Wu, Zi-Yi Song, Hong-Zhao Lu, Cheng-Chuang Song, Qiang-Ling Zhang, Jia-Yu Zhu, Gong-She Yang and Xin-E Shi *

Fuente: http://mdpi.com/


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