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1

School of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing 210046, Jiangsu, China

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Jiangsu Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials IAM, Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210046, Jiangsu, China

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Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China





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Abstract Quorum sensing QS has been recognized as a general phenomenon in microorganisms and plays an important role in many pathogenic bacteria. In this report, we used the Agrobacterium tumefaciens biosensor strain NT1 to rapidly screen for autoinducer-quenching inhibitors from bacteria. After initial screening 5389 isolates obtained from land and beach soil, 53 putative positive strains were identified. A confirmatory bioassay was carried out after concentrating the putative positive culture supernatant, and 22 strains were confirmed to have anti-LasR activity. Finally, we determined the strain JM2, which could completely inhibit biofilm formation of Pseudomonas aeruginosa PAO1, belonged to the genus Pseudomonas by analysis of 16S rDNA. Partially purified inhibitor factors F5 derived from culture supernatants specifically inhibited LasR-controlled elastase and protease in wild type P. aeruginosa PAO1 by 68% and 73%, respectively, without significantly affecting growth; the rhl-controlled pyocyanin and rhamnolipids were inhibited by 54% and 52% in the presence of 100 µg-mL of F5. The swarming motility and biofilm of PAO1 were also inhibited by F5. Real time RT-PCR on samples from 100 µg-mL F5-treated P. aeruginosa showed downregulation of autoinducer synthase LasRI and rhlI and cognate receptor lasR and rhlR genes by 50%, 28%, 48%, and 29%, respectively. These results provide compelling evidence that the F5 inhibitors interferes with the las system and significantly inhibits biofilm formation. View Full-Text

Keywords: quorum sensing; Pseudomonas aeruginosa; Agrobacterium tumefaciens NT1; biofilm; virulence factor quorum sensing; Pseudomonas aeruginosa; Agrobacterium tumefaciens NT1; biofilm; virulence factor





Autor: Lixing Weng 1,* , Yuqian Zhang 2, Yuxiang Yang 3 and Lianhui Wang 2,*

Fuente: http://mdpi.com/



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