Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative ActivityReportar como inadecuado




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College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China





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Abstract For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction SF. SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol CHOL, brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species ROS assays, and malondialdehyde MDA tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor NGF mimic activities again confirmed their neuroprotective function. View Full-Text

Keywords: neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer’s disease neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer’s disease





Autor: Yujuan Sun, Yanfei Lin, Xueli Cao, Lan Xiang * and Jianhua Qi *

Fuente: http://mdpi.com/



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