Therapeutic Effect of Activated Carbon-Induced Constipation Mice with Lactobacillus fermentum Suo on TreatmentReportar como inadecuado




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1

College of Food Science, Southwest University, Chongqing 400715, China

2

Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China

3

Science and Technology Administration, Chinese Academy of Agricultural Sciences, Biejing 100081, China

4

Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract The aim of this study was to investigate the effects of Lactobacillus fermentum Suo LF-Suo on activated carbon-induced constipation in ICR Institute of Cancer Research mice. ICR mice were orally administered with lactic acid bacteria for 9 days. Body weight, diet intake, drinking amount, defecation status, gastrointestinal transit and defecation time, and the serum levels of MTL motilin, Gas gastrin, ET endothelin, SS somatostatin, AChE acetylcholinesterase, SP substance P, VIP vasoactive intestinal peptide were used to evaluate the preventive effects of LF-Suo on constipation. Bisacodyl, a laxative drug, was used as a positive control. The normal, control, 100 mg-kg bisacodyl treatment, LB Lactobacillus bulgaricus-, LF-Suo L- and LF-Suo H-treated mice showed the time to the first black stool defecation at 90, 218, 117, 180, 155 and 137 min, respectively. By the oral administration of LB-, LF-Suo L, LF-Suo H or bisacodyl 100 mg-kg, the gastrointestinal transit was reduced to 55.2%, 72.3%, 85.5% and 94.6%, respectively, of the transit in normal mice, respectively. In contrast to the control mice, the serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Suo p < 0.05. By the RT-PCR reverse transcription–polymerase chain reaction and western blot assays, LF-Suo increased the c-Kit, SCF stem cell factor, GDNF glial cell line-derived neurotrophic factor and decreased TRPV1 transient receptor potential vanilloid 1, NOS nitric oxide synthase expressions of small intestine tissue in mice. These results demonstrate that lactic acid bacteria has preventive effects on mouse constipation and LF-Suo demonstrated the best functional activity. View Full-Text

Keywords: Lactobacillus fermentum Suo; activated carbon; constipation; bisacodyl; gastrointestinal transit Lactobacillus fermentum Suo; activated carbon; constipation; bisacodyl; gastrointestinal transit





Autor: Huayi Suo 1,†, Xin Zhao 2,†, Yu Qian 2,†, Guijie Li 2, Zhenhu Liu 3, Jie Xie 1 and Jian Li 4,*

Fuente: http://mdpi.com/



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