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College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China





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Abstract We have constructed three bacterial artificial chromosome BAC libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction PCR with the same successful rate as the universal 6D pooling strategy. View Full-Text

Keywords: bacterial artificial chromosome; wheat; descending pool bacterial artificial chromosome; wheat; descending pool





Autor: Wenjin Cao, Bisheng Fu, Kun Wu, Na Li, Yan Zhou, Zhongxia Gao, Musen Lin, Guoqiang Li, Xinyi Wu, Zhengqiang Ma and Haiyan Jia *

Fuente: http://mdpi.com/



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