Identification of a Plastid-Localized Bifunctional Nerolidol-Linalool Synthase in Relation to Linalool Biosynthesis in Young Grape BerriesReportar como inadecuado




Identification of a Plastid-Localized Bifunctional Nerolidol-Linalool Synthase in Relation to Linalool Biosynthesis in Young Grape Berries - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Centre for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

2

Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China

3

Qingdao Huadong Winery Co., Ltd., Northside of Lisha Road, Laoshan District, Qingdao 266000, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Monoterpenoids are a diverse class of natural products and contribute to the important varietal aroma of certain Vitis vinifera grape cultivars. Among the typical monoterpenoids, linalool exists in almost all grape varieties. A gene coding for a nerolidol-linalool NES-LINS synthase was evaluated in the role of linalool biosynthesis in grape berries. Enzyme activity assay of this recombinant protein revealed that it could convert geranyl diphosphate and farnesyl diphosphate into linalool and nerolidol in vitro, respectively, and thus it was named VvRILinNer. However, localization experiment showed that this enzyme was only localized to chloroplasts, which indicates that VvRILinNer functions in the linalool production in vivo. The patterns of gene expression and linalool accumulation were analyzed in the berries of three grape cultivars -Riesling- -Cabernet Sauvignon- -Gewurztraminer- with significantly different levels of monoterpenoids. The VvRILinNer was considered to be mainly responsible for the synthesis of linalool at the early developmental stage. This finding has provided us with new knowledge to uncover the complex monoterpene biosynthesis in grapes. View Full-Text

Keywords: grape berries; linalool synthase; subcellular localization; gene expression grape berries; linalool synthase; subcellular localization; gene expression





Autor: Bao-Qing Zhu 1,2,†, Jian Cai 1,†, Zhi-Qun Wang 1,3,†, Xiao-Qing Xu 1, Chang-Qing Duan 1 and Qiu-Hong Pan 1,*

Fuente: http://mdpi.com/



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