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1

School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China

2

Department of Silkworm Pathology, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China





*

Author to whom correspondence should be addressed.



Abstract Small ubiquitin-like modifier SUMO modification SUMOylation is an important and widely used reversible modification system in eukaryotic cells. It regulates various cell processes, including protein targeting, transcriptional regulation, signal transduction, and cell division. To understand its role in the model lepidoptera insect Bombyx mori, a recombinant baculovirus was constructed to express an enhanced green fluorescent protein eGFP-SUMO fusion protein along with ubiquitin carrier protein 9 of Bombyx mori BmUBC9. SUMOylation substrates from Bombyx mori cells infected with this baculovirus were isolated by immunoprecipitation and identified by LC–ESI-MS-MS. A total of 68 candidate SUMOylated proteins were identified, of which 59 proteins were functionally categorized to gene ontology GO terms. Analysis of kyoto encyclopedia of genes and genomes KEGG pathways showed that 46 of the identified proteins were involved in 76 pathways that mainly play a role in metabolism, spliceosome and ribosome functions, and in RNA transport. Furthermore, SUMOylation of four candidates polyubiquitin-C-like isoform X1, 3-hydroxyacyl-CoA dehydrogenase, cyclin-related protein FAM58A-like and GTP-binding nuclear protein Ran were verified by co-immunoprecipitation in Drosophila schneide 2 cells. In addition, 74% of the identified proteins were predicted to have at least one SUMOylation site. The data presented here shed light on the crucial process of protein sumoylation in Bombyx mori. View Full-Text

Keywords: SUMO; SUMOylation; Bombyx mori; LC–ESI-MS-MS; baculovirus SUMO; SUMOylation; Bombyx mori; LC–ESI-MS-MS; baculovirus





Autor: Xudong Tang 1,2,* , Xuliang Fu 1, Bifang Hao 1,2, Feng Zhu 1, Shengyan Xiao 1, Li Xu 1,2 and Zhongyuan Shen 1,2

Fuente: http://mdpi.com/



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