Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Trishydroxymethylphosphine as an Optional Coupling AgentReportar como inadecuado




Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Trishydroxymethylphosphine as an Optional Coupling Agent - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Department of Bioengineering, Tatung University, Taipei 104, Taiwan





*

Author to whom correspondence should be addressed.



Academic Editor: Hitoshi Sashiwa

Abstract β-Galactosidase was immobilized on chitosan-coated magnetic Fe3O4 nanoparticles and was used to produce galactooligosaccharides GOS from lactose. Immobilized enzyme was prepared with or without the coupling agent, trishydroxymethylphosphine THP. The two immobilized systems and the free enzyme achieved their maximum activity at pH 6.0 with an optimal temperature of 50 °C. The immobilized enzymes showed higher activities at a wider range of temperatures and pH. Furthermore, the immobilized enzyme coupled with THP showed higher thermal stability than that without THP. However, activity retention of batchwise reactions was similar for both immobilized systems. All the three enzyme systems produced GOS compound with similar concentration profiles, with a maximum GOS yield of 50.5% from 36% w·v−1 lactose on a dry weight basis. The chitosan-coated magnetic Fe3O4 nanoparticles can be regenerated using a desorption-re-adsorption process described in this study. View Full-Text

Keywords: chitosan; enzyme immobilization; Fe3O4 nanoparticles; β-galactosidase; galactooligosaccharides; THP chitosan; enzyme immobilization; Fe3O4 nanoparticles; β-galactosidase; galactooligosaccharides; THP





Autor: Su-Ching Chen and Kow-Jen Duan *

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados