The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic BalanceReportar como inadecuado




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1

Microelement Research Center, Key Laboratory of Arable Land Conservation Middle and Lower Reaches of Yangtze River, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China

2

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China





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Author to whom correspondence should be addressed.



Academic Editor: Hatem Rouached

Abstract Glutamine synthetase 2 GS2 is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate the chlorosis mechanism, we systematically analyzed the plant growth, carbon-nitrogen metabolism and gene expressions between the GS2-cosuppressed rice and wild-type plants. The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon-nitrogen ratio in the stems. Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage. The analysis of the metabolic profile showed a significant increase of sugars and organic acids. Additionally, gene expression patterns were different in root and leaf of GS2-cosuppressed plants between the seedling and tillering stage. These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth. View Full-Text

Keywords: carbon-nitrogen; chlorosis; gene expression; GS2; metabolic profile; rice carbon-nitrogen; chlorosis; gene expression; GS2; metabolic profile; rice





Autor: Aili Bao 1, Zhuqing Zhao 1, Guangda Ding 1, Lei Shi 1,2, Fangsen Xu 1,2 and Hongmei Cai 1,*

Fuente: http://mdpi.com/



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