Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor CharacteristicsReportar como inadecuado




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1

Julius Wolff Institute, Center for Musculoskeletal Surgery, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany

2

Berlin-Brandenburg Center for Regenerative Therapies, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany





*

Author to whom correspondence should be addressed.



Academic Editor: Christo Z. Christov

Abstract An imbalance between matrix metalloproteases MMPs and the tissue inhibitors of metalloproteases TIMPs may have a negative impact on the healing of rotator cuff tears. The aim of the project was to assess a possible relationship between clinical and radiographic characteristics of patients such as the age, sex, as well as the degenerative status of the tendon and the MMPs and TIMPs in their tenocyte-like cells TLCs. TLCs were isolated from ruptured supraspinatus tendons and quantitative Real-Time PCR and ELISA was performed to analyze the expression and secretion of MMPs and TIMPs. In the present study, MMPs, mostly gelatinases and collagenases such as MMP-2 -9 and -13 showed an increased expression and protein secretion in TLCs of donors with higher age or degenerative status of the tendon. Furthermore, the expression and secretion of TIMP-1 -2 and -3 was enhanced with age, muscle fatty infiltration and tear size. The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs. This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies. View Full-Text

Keywords: tenocytes; rotator cuff; age; degeneration; MMPs; TIMPs tenocytes; rotator cuff; age; degeneration; MMPs; TIMPs





Autor: Franka Klatte-Schulz 1,2,* , Thomas Aleyt 1,2, Stephan Pauly 1, Sven Geißler 1,2, Christian Gerhardt 1, Markus Scheibel 1 and Britt Wildemann 1,2

Fuente: http://mdpi.com/



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