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Analiza, Inc., Cleveland, OH 44114, USA

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Department of Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH 44115, USA

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Department of Chemistry, Philipps University, Marburg D-35032, Germany

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Department of Molecular Medicine and USF Health Byrd Alzheimers Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA

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Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia

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Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia

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Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia





*

Author to whom correspondence should be addressed.



Academic Editor: Malcolm D’Souza

Abstract The phase-transition temperatures of an elastin-like polypeptide ELP with the GVGVP40 sequence and solvent dipolarity-polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts Na2SO4, NaCl, NaClO4, and NaSCN and various osmolytes sucrose, sorbitol, trehalose, and trimethylamine N-oxide TMAO. All osmolytes decreased the ELP phase-transition temperature, whereas NaCl and Na2SO4 decreased, and NaSCN and NaClO4 increased it. The determined phase-transition temperatures may be described as a linear combination of the solvent’s dipolarity-polarizability and hydrogen-bond donor acidity. The linear relationship established for the phase-transition temperature in the presence of salts differs quantitatively from that in the presence of osmolytes, in agreement with different direct and indirect mechanisms of the influence of salts and osmolytes on the ELP phase-transition temperature. View Full-Text

Keywords: elastin-like polypeptide; phase-transition temperature; solvent properties; solvent dipolarity-polarizability; hydrogen-bond donor acidity; and hydrogen-bond acceptor basicity; osmolyte elastin-like polypeptide; phase-transition temperature; solvent properties; solvent dipolarity-polarizability; hydrogen-bond donor acidity; and hydrogen-bond acceptor basicity; osmolyte





Autor: Luisa A. Ferreira 1, James T. Cole 2, Christian Reichardt 3, Nolan B. Holland 2, Vladimir N. Uversky 4,5,6,7 and Boris Y. Zaslavsky 1,*

Fuente: http://mdpi.com/



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