Domain Motions and Functionally-Key Residues of l-Alanine Dehydrogenase Revealed by an Elastic Network ModelReportar como inadecuado




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College of Science, Yanshan University, Qinhuangdao 066004, China





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Academic Editor: Christo Christov

Abstract Mycobacterium tuberculosis l-alanine dehydrogenase l-MtAlaDH plays an important role in catalyzing l-alanine to ammonia and pyruvate, which has been considered to be a potential target for tuberculosis treatment. In the present work, the functional domain motions encoded in the structure of l-MtAlaDH were investigated by using the Gaussian network model GNM and the anisotropy network model ANM. The slowest modes for the open-apo and closed-holo structures of the enzyme show that the domain motions have a common hinge axis centered in residues Met133 and Met301. Accompanying the conformational transition, both the 1,4-dihydronicotinamide adenine dinucleotide NAD-binding domain NBD and the substrate-binding domain SBD move in a highly coupled way. The first three slowest modes of ANM exhibit the open-closed, rotation and twist motions of l-MtAlaDH, respectively. The calculation of the fast modes reveals the residues responsible for the stability of the protein, and some of them are involved in the interaction with the ligand. Then, the functionally-important residues relevant to the binding of the ligand were identified by using a thermodynamic method. Our computational results are consistent with the experimental data, which will help us to understand the physical mechanism for the function of l-MtAlaDH. View Full-Text

Keywords: Mycobacterium tuberculosis l-alanine dehydrogenase; domain motions; functionally-key residues; Gaussian network model; anisotropy network model; thermodynamic cycle method Mycobacterium tuberculosis l-alanine dehydrogenase; domain motions; functionally-key residues; Gaussian network model; anisotropy network model; thermodynamic cycle method





Autor: Xing-Yuan Li, Jing-Chao Zhang * , Yan-Ying Zhu and Ji-Guo Su *

Fuente: http://mdpi.com/



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