miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensiiReportar como inadecuado




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1

Fishery College, Guangdong Ocean University, Zhanjiang 524025, China

2

Guangdong Technology Research Center for Pearl Aquaculture and Process, Guangdong Ocean University, Zhanjiang 524025, China





*

Authors to whom correspondence should be addressed.



Academic Editor: Constantinos Stathopoulos

Abstract miR-29a is a conserved miRNA that participates in bone formation and immune response in vertebrates. miR-29a of Pinctada martensii Pm-miR-29a was identified in the previous research though deep sequencing. In this report, the precise sequence of mature Pm-miR-29a was validated using miRNA rapid amplification of cDNA ends miR-RACE technology. The precursor sequence of Pm-miR-29a was predicted to have 87 bp. Stem loop qRT-PCR analysis showed that Pm-miR-29a was easily detected in all the tissues, although expressions in the mantle and gill were low. The microstructure showed the disrupted growth of the nacre after Pm-miR-29a over-expression, which was induced by mimic injection into P. martensii. Results of the target analysis indicated that neuropeptide Y receptor type 2 Y2R was the potential target of Pm-miR-29a. Meanwhile, Pm-miR-29a mimics could obviously inhibit the relative luciferase activity of the reporter containing 3′ UTR Untranslated Regions of the Y2R gene. Furthermore, the expression of Y2R was downregulated whereas expressions of interleukin 17 IL-17 and nuclear factor κB NF-κB were upregulated after Pm-miR-29a over-expression in the mantle and gill, thereby suggesting that Pm-miR-29a could activate the immune response of the pearl oyster. Results showed that Pm-miR-29a was involved in nacre formation and immune response by regulating Y2R in pearl oyster P. martensii. View Full-Text

Keywords: Pm-miR-29a; Pinctada martensii; nacre formation; biomineralization; immune response Pm-miR-29a; Pinctada martensii; nacre formation; biomineralization; immune response





Autor: Rongrong Tian 1, Zhe Zheng 1, Ronglian Huang 1, Yu Jiao 1,* and Xiaodong Du 1,2,*

Fuente: http://mdpi.com/



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