Protein Arginine Methyltransferase 6 Involved in Germ Cell Viability during Spermatogenesis and Down-Regulated by the Androgen ReceptorReportar como inadecuado




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1

Department of Physiology, Shantou University Medical College, Shantou 515041, China

2

Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, China

3

Department of Surgery, Guangzhou Medical University, Guangzhou 510182, China

4

Department of Surgery, Anhui Medical University, Hefei 230032, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Alan C. Leonard

Abstract Androgens and the androgen receptor AR are of great importance to spermatogenesis and male fertility. AR knockout ARKO mice display a complete insensitivity to androgens and male infertility; however, the exact molecular mechanism for this effect remains unclear. In this study, we found that the expression levels of Prmt6 mRNA and protein were significantly up-regulated in the testes of ARKO mice compared to wild type WT mice. PRMT6 was principally localized to the nucleus of spermatogonia and spermatocytes by immunofluorescence staining. Furthermore, luciferase assay data showed that AR together with testosterone treatment suppressed Prmt6 transcription via binding to the androgen-responsive element ARE of the Prmt6 promoter. Moreover, knockdown of Prmt6 suppressed germ cells migration and promoted apoptosis. In addition, both of these cellular activities could not be enhanced by testosterone treatment. Taken together, these data indicate that PRMT6, which was down-regulated by AR and influenced cell migration and apoptosis of germ cells, could play a potentially important role in spermatogenesis. View Full-Text

Keywords: PRMT6; androgen receptor; testes; migration; apoptosis PRMT6; androgen receptor; testes; migration; apoptosis





Autor: Manling Luo 1,†, Yuchi Li 1,2,†, Huan Guo 2,3, Shouren Lin 2, Jianbo Chen 2,4, Qian Ma 2, Yanli Gu 2, Zhimao Jiang 2 and Yaoting Gui 2,*

Fuente: http://mdpi.com/



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