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1

Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy

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Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019 Sesto Fiorentino, Italy

3

Department of Biology, Università di Firenze, via Micheli 1, 50121 Firenze, Italy





*

Author to whom correspondence should be addressed.



Academic Editor: Marcello Iriti

Abstract Cerato-platanin CP is the founder of a fungal protein family consisting in non-catalytic secreted proteins, which work as virulence factors and-or as elicitors of defense responses and systemic resistance, thus acting as PAMPs pathogen-associated molecular patterns. Moreover, CP has been defined an expansin-like protein showing the ability to weaken cellulose aggregates, like the canonical plant expansins do. Here, we deepen the knowledge on CP PAMP activity by the use of a multi-disciplinary approach: proteomic analysis, VOC volatile organic compound measurements, and gas exchange determination. The treatment of Arabidopsis with CP induces a differential profile either in protein expression or in VOC emission, as well changes in photosynthetic activity. In agreement with its role of defense activator, CP treatment induces down-expression of enzymes related to primary metabolism, such as RuBisCO, triosephosphate isomerase, and ATP-synthase, and reduces the photosynthesis rate. Conversely, CP increases expression of defense-related proteins and emission of some VOCs. Interestingly, CP exposure triggered the increase in enzymes involved in GSH metabolism and redox homeostasis glutathione S-transferase, thioredoxin, Cys-peroxiredoxin, catalase and in enzymes related to the -glucosinolate-myrosinase- system, which are the premise for synthesis of defence compounds, such as camalexin and some VOCs, respectively. The presented results are in agreement with the accepted role of CP as a PAMP and greatly increase the knowledge of plant primary defences induced by a purified fungal elicitor. View Full-Text

Keywords: cerato-platanin; expansin; PAMP; plant defense; ROS signaling; VOC accumulation cerato-platanin; expansin; PAMP; plant defense; ROS signaling; VOC accumulation





Autor: Simone Luti 1, Anna Caselli 1, Cosimo Taiti 2, Nadia Bazihizina 2, Cristina Gonnelli 3, Stefano Mancuso 2 and Luigia Pazzagli 1,*

Fuente: http://mdpi.com/



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