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Department of Biomedical Experimental and Clinical Sciences, Università di Firenze, viale Morgagni 50, 50134 Firenze, Italy


Department of Agri-Food and Environmental Science, Università di Firenze, via delle Idee 30, 50019 Sesto Fiorentino, Italy


Department of Biology, Università di Firenze, via Micheli 1, 50121 Firenze, Italy


Author to whom correspondence should be addressed.

Academic Editor: Marcello Iriti

Abstract Cerato-platanin CP is the founder of a fungal protein family consisting in non-catalytic secreted proteins, which work as virulence factors and-or as elicitors of defense responses and systemic resistance, thus acting as PAMPs pathogen-associated molecular patterns. Moreover, CP has been defined an expansin-like protein showing the ability to weaken cellulose aggregates, like the canonical plant expansins do. Here, we deepen the knowledge on CP PAMP activity by the use of a multi-disciplinary approach: proteomic analysis, VOC volatile organic compound measurements, and gas exchange determination. The treatment of Arabidopsis with CP induces a differential profile either in protein expression or in VOC emission, as well changes in photosynthetic activity. In agreement with its role of defense activator, CP treatment induces down-expression of enzymes related to primary metabolism, such as RuBisCO, triosephosphate isomerase, and ATP-synthase, and reduces the photosynthesis rate. Conversely, CP increases expression of defense-related proteins and emission of some VOCs. Interestingly, CP exposure triggered the increase in enzymes involved in GSH metabolism and redox homeostasis glutathione S-transferase, thioredoxin, Cys-peroxiredoxin, catalase and in enzymes related to the -glucosinolate-myrosinase- system, which are the premise for synthesis of defence compounds, such as camalexin and some VOCs, respectively. The presented results are in agreement with the accepted role of CP as a PAMP and greatly increase the knowledge of plant primary defences induced by a purified fungal elicitor. View Full-Text

Keywords: cerato-platanin; expansin; PAMP; plant defense; ROS signaling; VOC accumulation cerato-platanin; expansin; PAMP; plant defense; ROS signaling; VOC accumulation

Autor: Simone Luti 1, Anna Caselli 1, Cosimo Taiti 2, Nadia Bazihizina 2, Cristina Gonnelli 3, Stefano Mancuso 2 and Luigia Pazzagli 1,*



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