Molecular Characterization of the Complete Genome of Three Basal-BR Isolates of Turnip mosaic virus Infecting Raphanus sativus in ChinaReportar como inadecuado




Molecular Characterization of the Complete Genome of Three Basal-BR Isolates of Turnip mosaic virus Infecting Raphanus sativus in China - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

College of Plant Sciences, Jilin University, Changchun 130062, China



These authors contributed equally to this work.





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Academic Editor: Jianhua Zhu

Abstract Turnip mosaic virus TuMV infects crops of plant species in the family Brassicaceae worldwide. TuMV isolates were clustered to five lineages corresponding to basal-B, basal-BR, Asian-BR, world-B and OMs. Here, we determined the complete genome sequences of three TuMV basal-BR isolates infecting radish from Shandong and Jilin Provinces in China. Their genomes were all composed of 9833 nucleotides, excluding the 3′-terminal polyA tail. They contained two open reading frames ORFs, with the large one encoding a polyprotein of 3164 amino acids and the small overlapping ORF encoding a PIPO protein of 61 amino acids, which contained the typically conserved motifs found in members of the genus Potyvirus. In pairwise comparison with 30 other TuMV genome sequences, these three isolates shared their highest identities with isolates from Eurasian countries Germany, Italy, Turkey and China. Recombination analysis showed that the three isolates in this study had no -clear- recombination. The analyses of conserved amino acids changed between groups showed that the codons in the TuMV out group OGp and OMs group were the same at three codon sites 852, 1006, 1548, and the other TuMV groups basal-B, basal-BR, Asian-BR, world-B were different. This pattern suggests that the codon in the OMs progenitor did not change but that in the other TuMV groups the progenitor sequence did change at divergence. Genetic diversity analyses indicate that the PIPO gene was under the highest selection pressure and the selection pressure on P3N-PIPO and P3 was almost the same. It suggests that most of the selection pressure on P3 was probably imposed through P3N-PIPO. View Full-Text

Keywords: TuMV; complete genome sequence; basal-BR; genetic diversity; Raphanus sativus TuMV; complete genome sequence; basal-BR; genetic diversity; Raphanus sativus





Autor: Fuxiang Zhu †, Ying Sun †, Yan Wang, Hongyu Pan, Fengting Wang, Xianghui Zhang, Yanhua Zhang and Jinliang Liu *

Fuente: http://mdpi.com/



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