The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K-Akt Signaling PathwayReportar como inadecuado




The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K-Akt Signaling Pathway - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Department of Ophthalmology, Second Hospital of Jilin University, Jilin University, Changchun 130041, China

2

Department of Ophthalmology, China-Japan Union Hospital, Jilin University, Changchun 130031, China





*

Author to whom correspondence should be addressed.



Academic Editor: Atsushi Matsuzawa

Abstract Recent evidence suggests that chloride channels are critical for cell proliferation, migration, and differentiation. We examined the effects of transforming growth factor TGF-β1 on chloride channel expression and associations with human conjunctival fibroblast HConF biology. To investigate the potential role of chloride channel CLC-2 in migration, transition to myofibroblasts and extracellular matrix ECM synthesis of HconF, a small interfering RNA siRNA approach was applied. TGF-β1-induced migration and transition of fibroblasts to myofibroblasts characterized by α-smooth muscle actin α-SMA expression, supported by increased endogenous expression of CLC-2 protein and mRNA transcripts. ECM collagen I and fibronectin synthesis in HConF was enhanced by TGF-β1. CLC-2 siRNA treatment reduced TGF-β1-induced cell migration, transition of fibroblasts to myofibroblasts, and ECM synthesis of HConF. CLC-2 siRNA treatment in the presence of TGF-β1 inhibited phosphorylation of PI3K and Akt in HConF. These findings demonstrate that CLC-2 chloride channels are important for TGF-β1-induced migration, differentiation, and ECM synthesis via PI3K-Akt signaling in HConF. View Full-Text

Keywords: CLC-2 siRNA; TGF-β1 differentiation; extracellular matrix; human conjunctival fibroblasts; migration; signaling pathway CLC-2 siRNA; TGF-β1 differentiation; extracellular matrix; human conjunctival fibroblasts; migration; signaling pathway





Autor: Lixia Sun 1, Yaru Dong 1, Jing Zhao 1,2, Yuan Yin 1 and Yajuan Zheng 1,*

Fuente: http://mdpi.com/



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