Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal ModelReportar como inadecuado


Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model


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1

College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea

2

Department of Obstetrics & Gynecology, Daejeon St. Mary’s Hospital, The Catholic University of Korea, 64 Daeheung-Ro Daeheung-dong, Jung-gu, Daejeon 301-723, Korea

3

Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg 411-15, Sweden





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Academic Editor: Allison Cowin

Abstract Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica TECA in a phthalic anhydride PA-induced atopic dermatitis AD animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos:HR-1 mouse. After AD induction, 100 μL of 0.2% and 0.4% of TECA 40 μg or 80 μg-cm2 was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase iNOS, cyclooxygenase-2 COX-2, and NF-κB activity, which were determined by electromobility shift assay EMSA. We also measured TNF-α, IL-1β, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay ELISA. TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-κB activity as well as the release of TNF-α, IL-1β, IL-6, and IgE. In addition, TECA 1, 2, 5 μg-mL potently inhibited Lipopolysaccharide LPS 1 μg-mL-induced NO production, expression of iNOS and COX-2, and NF-κB DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-κB signaling. View Full-Text

Keywords: titrated extract of Centella asiatica; skin inflammation; atopic dermatitis; NF-κB; cytokine; IgE titrated extract of Centella asiatica; skin inflammation; atopic dermatitis; NF-κB; cytokine; IgE





Autor: Ju Ho Park 1, Ji Yeon Choi 1, Dong Ju Son 1, Eun Kyung Park 2, Min Jong Song 2, Mats Hellström 3 and Jin Tae Hong 1,*

Fuente: http://mdpi.com/



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