Genotypes and Antibiotic Resistances of Campylobacter jejuni Isolates from Cattle and Pigeons in Dairy FarmsReportar como inadecuado




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1

Istituto Zooprofilattico Sperimentale della Lombardia e dellEmilia Romagna, Lodi 26900, Italy

2

Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Putignano BA 70017, Italy

3

Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern CH-3001, Switzerland





*

Author to whom correspondence should be addressed.



Abstract Campylobacter jejuni is the most common food-borne zoonotic pathogen causing human gastroenteritis worldwide and has assumed more importance in Italy following the increased consumption of raw milk. Our objectives were to get an overview of genotypes and antibiotic resistances in C. jejuni isolated from milk, cattle feces, and pigeons in dairy herds of Northern Italy. flaB-typing was applied to 78 C. jejuni isolates, previously characterized by Multi-Locus Sequence Typing, and genotypic resistances towards macrolides and quinolones based on point mutations in the 23S rRNA and gyrA genes, respectively, were determined. flaB-typing revealed 22 different types with one of them being novel and was useful to further differentiate strains with an identical Sequence Type ST and to identify a pigeon-specific clone. Macrolide resistance was not found, while quinolone resistance was detected in 23.3% of isolates. A relationship between specific genotypes and antibiotic resistance was observed, but was only significant for the Clonal Complex 206. Our data confirm that pigeons do not play a role in the spread of C. jejuni among cattle and they are not responsible for milk contamination. A relevant number of bulk milk samples were contaminated by C. jejuni resistant to quinolones, representing a possible source of human resistant strains. View Full-Text

Keywords: Campylobacter jejuni; dairy farms; pigeons; genotyping; antibiotic resistance Campylobacter jejuni; dairy farms; pigeons; genotyping; antibiotic resistance





Autor: Valentina Bianchini 1, Mario Luini 1,* , Laura Borella 1, Antonio Parisi 2, Romie Jonas 3, Sonja Kittl 3 and Peter Kuhnert 3

Fuente: http://mdpi.com/



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