Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 CellsReportar como inadecuado




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1

Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada

2

Centre for Bone and Muscle Health, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada

3

Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editors: Marlena Kruger and Hope Weiler

Abstract Flavonoid intake is positively correlated to bone mineral density BMD in women. Flavonoids such as quercetin exhibit strong anti-oxidant and anti-inflammatory activity that may be beneficial for bone health. Quercetin, previously shown to positively influence osteoblasts, is metabolized into glycosides including rutin and hyperoside. We compared the effects of these glycosides on mineralization in human osteoblast Saos2 cells. Administration of rutin ≥25 µM and hyperoside ≥5 µM resulted in higher mineral content, determined using the alizarin red assay. This was accompanied by higher alkaline phosphatase activity with no cell toxicity. The expression of osteopontin, sclerostin, TNFα and IL6, known stimuli for decreasing osteoblast activity, were reduced with the addition of rutin or hyperoside. In summary, rutin and hyperoside require supraphysiological levels, when administered individually, to positively influence osteoblast activity. This information may be useful in developing nutraceuticals to support bone health. View Full-Text

Keywords: flavonoids; hyperoside; mineral; rutin; osteoblasts; tea flavonoids; hyperoside; mineral; rutin; osteoblasts; tea





Autor: Leslie A. Nash 1,2, Sandra J. Peters 2,3,†, Philip J. Sullivan 3,† and Wendy E. Ward 1,2,3,*

Fuente: http://mdpi.com/



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