Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B CellsReportar como inadecuado




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Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China





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Academic Editor: Paul B. Tchounwou

Abstract The aim of the present study was to investigate the effects of size-fractionated i.e., <1; 1–2.5, and 2.5–10 µm in an aerodynamic diameter ambient particulate matter PM on reactive oxygen species ROS activity and cell viability in human bronchial epithelial cells BEAS-2B. The PM samples were collected from an urban site uPM in Beijing and a steel factory site sPM in Anshan, China, from March 2013 to December 2014. Metal elements, organic and elemental carbon, and water-soluble inorganic ions in the uPM and sPM were analyzed. The cell viability and ROS generation in PM-exposed BEAS-2B cells were measured by MTS and DCFH-DA. The results showed that both uPM and sPM caused a decrease in the cell viability and an increase in ROS generation. The level of ROS measured in sPM1.0 was approximately triple that in uPM1.0. The results of correlation analysis showed that the ROS activity and cytotoxicity were related to different PM composition. Moreover, deferoxamine DFO significantly prevented the increase of ROS generation and the decrease of cell viability. Taken together, our results suggest that the metals absorbed on PM induced oxidant radical generation in BEAS-2B cells that could lead to impairment of pulmonary function. View Full-Text

Keywords: particulate matter; size-fraction; chemical composition; correlation analysis; cytotoxicity particulate matter; size-fraction; chemical composition; correlation analysis; cytotoxicity





Autor: Longfei Guan, Wei Rui, Ru Bai, Wei Zhang, Fang Zhang * and Wenjun Ding *

Fuente: http://mdpi.com/



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