Molecular Mechanism Underlying the Entomotoxic Effect of Colocasia esculenta Tuber Agglutinin against Dysdercus cingulatusReportar como inadecuado




Molecular Mechanism Underlying the Entomotoxic Effect of Colocasia esculenta Tuber Agglutinin against Dysdercus cingulatus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Division of Plant Biology, Bose Institute, Centenary Campus, P1-12, CIT Scheme, VIIM, Kakurgachi, Kolkata 700054, West Bengal, India





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Academic Editor: Brian T. Forschler

Abstract Colocasia esculenta tuber agglutinin CEA, a mannose binding lectin, exhibits insecticidal efficacy against different hemipteran pests. Dysdercus cingulatus, red cotton bug RCB, has also shown significant susceptibility to CEA intoxication. However, the molecular basis behind such entomotoxicity of CEA has not been addressed adequately. The present study elucidates the mechanism of insecticidal efficacy of CEA against RCB. Confocal and scanning electron microscopic analyses documented CEA binding to insect midgut tissue, resulting in an alteration of perimicrovillar membrane PMM morphology. Internalization of CEA into insect haemolymph and ovary was documented by western blotting analyses. Ligand blot followed by mass spectrometric identification revealed the cognate binding partners of CEA as actin, ATPase and cytochrome P450. Deglycosylation and mannose inhibition assays indicated the interaction to probably be mannose mediated. Bioinformatic identification of putative glycosylation or mannosylation sites in the binding partners further supports the sugar mediated interaction. Correlating entomotoxicity of CEA with immune histological and binding assays to the insect gut contributes to a better understanding of the insecticidal potential of CEA and endorses its future biotechnological application. View Full-Text

Keywords: Colocasia esculenta tuber agglutinin; hemipteran pest; MALDI TOF-TOF; perimicrovillar membrane PMM; entomotoxicity Colocasia esculenta tuber agglutinin; hemipteran pest; MALDI TOF-TOF; perimicrovillar membrane PMM; entomotoxicity





Autor: Amit Roy # and Sampa Das *

Fuente: http://mdpi.com/



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