Review of the Application of Modern Cytogenetic Methods FISH-GISH to the Study of Reticulation Polyploidy-HybridisationReportar como inadecuado




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1

Department of Biology, University of Florida, Gainesville, Florida 32611, USA

2

School of Biological and Chemical Sciences, Queen Mary, University of London, UK

3

Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611, USA





*

Author to whom correspondence should be addressed.



Abstract The convergence of distinct lineages upon interspecific hybridisation, including when accompanied by increases in ploidy allopolyploidy, is a driving force in the origin of many plant species. In plant breeding too, both interspecific hybridisation and allopolyploidy are important because they facilitate introgression of alien DNA into breeding lines enabling the introduction of novel characters. Here we review how fluorescence in situ hybridisation FISH and genomic in situ hybridisation GISH have been applied to: 1 studies of interspecific hybridisation and polyploidy in nature, 2 analyses of phylogenetic relationships between species, 3 genetic mapping and 4 analysis of plant breeding materials. We also review how FISH is poised to take advantage of nextgeneration sequencing NGS technologies, helping the rapid characterisation of the repetitive fractions of a genome in natural populations and agricultural plants. View Full-Text

Keywords: cytogenetics; ISH; polyploidy cytogenetics; ISH; polyploidy





Autor: Michael Chester 1, Andrew R. Leitch 2,* , Pamela S. Soltis 3 and Douglas E. Soltis 1

Fuente: http://mdpi.com/



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