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1

CLC bio, 8200 Aarhus N, Denmark

2

Department of Biology, Box 118525, University of Florida, Gainesville, Florida, 32611-8525, USA





*

Author to whom correspondence should be addressed.



Abstract This study presents a new computer program for assessing the effects of different factors and sequencing strategies on de novo sequence assembly. The program uses reads from actual sequencing studies or from simulations with a reference genome that may also be real or simulated. The simulated reads can be created with our read simulator. They can be of differing length and coverage, consist of paired reads with varying distance, and include sequencing errors such as color space miscalls to imitate SOLiD data. The simulated or real reads are mapped to their reference genome and our assembly simulator is then used to obtain optimal assemblies that are limited only by the distribution of repeats. By way of this mapping, the assembly simulator determines which contigs are theoretically possible, or conversely and perhaps more importantly, which are not. We illustrate the application and utility of our new simulation tools with several experiments that test the effects of genome complexity repeats, read length and coverage, word size in De Bruijn graph assembly, and alternative sequencing strategies e.g., BAC pooling on sequence assemblies. These experiments highlight just some of the uses of our simulators in the experimental design of sequencing projects and in the further development of assembly algorithms. View Full-Text

Keywords: assembly simulator; read simulator; de novo assembly; sequencing strategies; next generation sequencing assembly simulator; read simulator; de novo assembly; sequencing strategies; next generation sequencing





Autor: Bjarne Knudsen 1, Roald Forsberg 1 and Michael M. Miyamoto 2,*

Fuente: http://mdpi.com/



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