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Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA





Academic Editor: A. Sheila Holmes-Smith

Abstract Total internal reflection fluorescence TIRF microscopy has been widely used as a single molecule imaging technique to study various fundamental aspects of cell biology, owing to its ability to selectively excite a very thin fluorescent volume immediately above the substrate on which the cells are grown. However, TIRF microscopy has found little use in high content screening due to its complexity in instrumental setup and experimental procedures. Inspired by the recent demonstration of label-free evanescent wave biosensors for cell phenotypic profiling and drug screening with high throughput, we had hypothesized and demonstrated that TIRF imaging is also amenable to receptor pharmacology profiling. This paper reviews key considerations and recent applications of TIRF imaging for pharmacology profiling. View Full-Text

Keywords: evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence





Autor: Ye Fang

Fuente: http://mdpi.com/



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