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Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA





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Academic Editor: Jeff D. Newman

Abstract We have developed an optofluidic biosensor to study microscale particles and different species of microalgae. The system is comprised of a microchannel with a set of chevron-shaped grooves. The chevrons allows for hydrodynamic focusing of the core stream in the center using a sheath fluid. The device is equipped with a new generation of highly sensitive photodetectors, multi-pixel photon counter MPPC, with high gain values and an extremely small footprint. Two different sizes of high intensity fluorescent microspheres and three different species of algae Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana were studied. The forward scattering emissions generated by samples passing through the interrogation region were carried through a multimode fiber, located in 135 degree with respect to the excitation fiber, and detected by a MPPC. The signal outputs obtained from each sample were collected using a data acquisition system and utilized for further statistical analysis. Larger particles or cells demonstrated larger peak height and width, and consequently larger peak area. The average signal output integral of the peak for Chlamydomonas reinhardtii strain 21 gr, Chlamydomonas suppressor, and Chlorella sorokiniana falls between the values found for the 3.2 and 10.2 μm beads. Different types of algae were also successfully characterized. View Full-Text

Keywords: optofluidics; microfluidics; multi-pixel photon counter optofluidics; microfluidics; multi-pixel photon counter





Autor: Pouya Asrar, Marta Sucur and Nastaran Hashemi *

Fuente: http://mdpi.com/



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